20220114 Re-QCing DNA samples from the E5 Project
Rechecking the DNA from the E5 project
Troubleshooting two coral species from each of the four timepoints
QC Date: January 14, 2022
Samples
| Tube number | Timepoint | Species | Colony ID | Extraction date |
|---|---|---|---|---|
| 7 | January | Porites | POR-262 | 20211105 |
| 13 | January | Porites | POR-245 | 20211105 |
| 109 | January | Porites | POR-341 | 20210902 |
| 125 | January | Porites | POR-355 | 20211108 |
| 167 | January | Porites | POR-383 | 20211112 |
| 173 | January | Porites | POR-349 | 20211129 |
| 191 | January | Porites | POR-71 | 20211115 |
| 193 | January | Porites | POR-75 | 20211129 |
| 195 | January | Porites | POR-69 | 20211130 |
| 209 | January | Porites | POR-79 | 20211130 |
| 235 | January | Porites | POR-72 | 20210903 |
| 267 | March | Pocillopora | POC-257 | 20211112 |
| 287 | March | Porites | POR-235 | 20211104 |
| 409 | March | Porites | POR-340 | 20210903 |
| 459 | March | Porites | POR-385 | 20211102 |
| 469 | March | Porites | POR-83 | 20211122 |
| 577 | Sept | Pocillopora | POC-377 | 20210903 |
| 601 | Sept | Porites | POR-362 | 20211112 |
| 647 | Sept | Porites | POR-71 | 20211115 |
| 795 | Nov | Porites | POR-354 | 20211105 |
| 831 | Nov | Porites | POR-349 | 20211118 |
QC notes
- These were all samples that had no detectable DNA using the Qubit BR kit, but had a band when run out on a gel
Qubit
- Used High Sensitivity DNA Qubit Protocol
- All samples read twice, standards only read once
DNA
| Tube number | RFU | DNA 1 (ng/uL) | DNA 2 (ng/uL) | Average |
|---|---|---|---|---|
| Standard 1 | 54.14 | |||
| Standard 2 | 24741.95 | |||
| 7 | 0.196 | 0.190 | 0.193 | |
| 13 | 0.218 | 0.172 | 0.195 | |
| 109 | 0.632 | 0.630 | 0.631 | |
| 125 | 0.308 | 0.224 | 0.276 | |
| 167 | 0.456 | 0.454 | 0.455 | |
| 173 | 0.366 | 0.352 | 0.359 | |
| 191 | 0.440 | 0.462 | 0.451 | |
| 193 | 0.430 | 0.436 | 0.433 | |
| 195 | nd | nd | nd | |
| 209 | 0.258 | 0.322 | 0.290 | |
| 235 | 0.638 | 0.646 | 0.642 | |
| 267 | 102.0 | 100.0 | 101.0 | |
| 287 | 0.792 | 0.760 | 0.776 | |
| 409 | 1.20 | 1.20 | 1.20 | |
| 459 | 0.302 | 0.304 | 0.303 | |
| 469 | 0.430 | 0.524 | 0.477 | |
| 577 | 10.8 | 10.8 | 10.8 | |
| 601 | 0.312 | 0.286 | 0.299 | |
| 647 | 0.762 | 0.754 | 0.758 | |
| 795 | 0.304 | 0.290 | 0.297 | |
| 831 | 1.39 | 1.22 | 1.305 |
Nanodrop
- Used ThermoScientific Nanodrop 2000
- Blanked using the Tris HCl
DNA
| Tube number | ng/uL | 260:230 |
|---|---|---|
| 7 | -0.8 | 1.09 |
| 13 | 3.1 | 1.46 |
| 109 | -0.4 | 0.49 |
| 125 | 0.4 | -4.63 |
| 167 | 0.9 | 3.58 |
| 173 | 1.3 | 2.70 |
| 191 | -0.1 | 0.39 |
| 193 | 0.3 | -1.15 |
| 195 | -0.3 | 0.46 |
| 209 | 0.7 | 1.54 |
| 235 | -0.5 | 0.95 |
| 267 | 127.3 | 1.84 |
| 287 | -1.0 | 1.02 |
| 409 | 0.4 | -1.21 |
| 459 | 0.7 | 2.16 |
| 469 | -0.9 | 3.24 |
| 577 | 12.5 | 1.94 |
| 601 | 2.4 | 2.08 |
| 647 | 0.8 | 1.22 |
| 795 | 2.9 | 1.42 |
| 831 | 3.2 | 1.80 |
Addtional Notes
- NA
Written on January 14, 2022