20221027 RNA DNA extractions from E5 project
DNA/RNA extractions from E5 project
20220929 RNA DNA extractions from E5 project
DNA/RNA extractions from E5 project
20220718 mtORF PCR and ethanol precipitation For C. Dube
mtORF PCR of Pocillopora spp for Caroline Dube
Loosely based off of this protocol.
20220426 RFPL and Bento Lab testing of POC E5 samples
Continuing to the test the RFLPs on POC samples from the E5 project. Looking to see if the RE match the BLAST results.
20220314 RFPL and Bento Lab testing
Pocillopora of Moorea has cryptic diversity, species cannot be discerned by morphological characteristics. Even by generating a molecular barcode (ATP synthase 6), there are some species that are identical and a different gene needs to be sequenced to correctly identify these species. It’s really important to be able to tell these different species apart to make sure enough samples from each species are collected. While generating these sequence barcodes isn’t difficult, it can’t easily be done in the field, but what if it was?
20220310 mtORF PCR and ethanol precipitation of Pocillopora
mtORF PCR of Pocillopora
Loosely based off of this protocol.
20220303 RNA DNA extractions for unknown coral fragments
DNA/RNA extractions from Putnam Lab collaborator
20220302 mtORF PCR and ethanol precipitation of Pocillopora
mtORF PCR of Pocillopora
Loosely based off of this protocol.